LAMP on Disc COVID-19 Test
Loop-mediated isothermal amplification (LAMP) based COVID-19 test.
The loop-mediated isothermal amplification (LAMP) based COVID-19 test uses viral RNA from heat-inactivated nasopharyngeal swab samples in an integrated modular centrifugal microfluidic platform. This method can deliver 20 test results via smartphone within 30 minutes. One device can perform up to 600 tests per day. This product is currently a prototype.
This product was selected for inclusion in WHO’s 2021 Compendium of Innovative Health Technologies for Low‐Resource Settings.
Africa, Americas, and Eastern Mediterranean regions
None, as of 2020
500 USD for technology, 10 USD for consumables
Goal 3: Good health and well-being
Locations with a need for lower cost COVID-19 testing.
1. Collect swab sample into a transportation media; 2. Heat at 95 for 5 min to inactivate the sample (virus is now dead); 3. Take 1 microliter sample and add to LAMP mastermix (total volume = 10 uL) and pipette the solution into the microfluidics Disc channel and seal with a tape (Each disc can take 20 different samples); 4 Put the disc into the Hardware and push a button to start the isothermal reaction (programmed to stop after 30 min);6. Take a picture using smartphone (and analyse the image)
Using state of the art microfluidics, we have developed a LAMP based COVID test. In addition to precise fluid control, at the heart of our innovation is a novel agarose bead-based sample processing strategy to (1) stop the reaction and (2) improve the signal response of reaction (approx. 30x signal enhancement), thus allowing either visual by naked eye or smartphone-based detection. 1 uL swab sample is added to LAMP mastermix –> heat at 65 for 30 min –> readout using any smartphone.
The end-user takes swab sample,add to Lamp mastermix and and put into the channel, and push start button. After the reaction, the end-user uses a smart phone to take an image for analysis. Since the reaction is terminated automatically, the enduser do not need to make the image analysis directly
Uses rechargeable batteries
1 kg, 250 x 200 x 150 mm3
Reusable, cost-effective, and scalable
The method was first tested using extracted SARS-CoV-2 RNA for testing the assay specificity(specificity: 100% for SARS-CoV-2). Following, using clinical swab samples from Karolinska Hospital, the method was benchmarked against RT-qPCR (n =31), with proven sensitivity of 94% and specificity of 100%. Note, while the RT-qPCR was performed in centralised lab using standard procedure, our method takes heat-inactivated clinical sample and deliver results within 30 minutes;
1) We are planning to perform two pilot studies at resource-limited settings over the coming 6 months (in Ethiopia and Nigeria).
KTH Royal Institute of Technology
Current testing at Karolinska University Hospital
Requires replaceable batteries to function as well as a clean water supply.
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